Goodjob-Capture Software-operation progress
捕捉軟件的操作流程
1:CEL image capture 化學(xué)發(fā)光影像的捕捉
The system will automatic active the image capture program when booting.
系統(tǒng)啟動(dòng)時(shí)，系統(tǒng)自動(dòng)運(yùn)行影像捕捉程序。
2:Quick user guide 快速使用指南
(1) choose Loop to start realtime image to show sample area and load chemi-membrance.
選擇“Loop”鍵，實(shí)時(shí)影像會顯示樣品面積和負(fù)載化學(xué)膜。
(2) Choose bin2 to speed up realtime image running. 選擇bin2，加快實(shí)時(shí)影像運(yùn)行
PS: For chemi-sample pre-set parameter 備注：化學(xué)發(fā)光樣品預(yù)先參數(shù)設(shè)置
1) auto-stretch atuo-stretch（自動(dòng)延伸）
2) negative negative(否定、負(fù)的)
(3) turn off the reflectant white light when start to capture image 當(dāng)開始影像捕捉時(shí)，關(guān)閉反射白光
(4) change exp.time 改變曝光時(shí)間
(5) press Loop again to stop realtime image .press Time-lapse,set desire capture number
再次按“Loop”鍵停止實(shí)時(shí)取相。按“Time-lapse”(延時(shí))，設(shè)置想要的捕捉圖片數(shù)量。
(6) choose directory to save capture images save as tif.must be key in this eaction is only first time use time-lapse. Routine use just press Next dir will auto save to new diretory.
選擇目錄，以tif.的后綴名保存照片。這一步在**次使用“Time lapse”時(shí)是非常關(guān)鍵的。常規(guī)應(yīng)用就可以直接選擇“Next dir”(下級目錄)，系統(tǒng)會自動(dòng)保存到新的地址。
PS:Must select “Autosave images”before capture. Otherwise the present directory will have no image.
備注：捕捉前必須選擇“Autosave images”，負(fù)責(zé)目前的目錄里不會存有圖像。
(7) start to capture image Time-lapse fuction. 開始用“Time-lapse”捕捉圖像。
(8) Under auto-stretch control in the beginning the display window will be high sensitive on weak bands and its image will be a little rough that depend on samples concentration.
在開始“auto-stretch”功能的作用下，窗口會出現(xiàn)高敏感度的弱信號條帶并且圖像有點(diǎn)粗糙，這都取決于樣品的濃度。
This is helpful for user to know whether weaks bands is there. It will show nice picture in the final images after more fluorescent signal collected.
這有助于使用者發(fā)現(xiàn)是否存有弱信號條帶。并且在收集更多熒光信號后，*后的成片效果會非常好。
Final images will display good signal intensity and have white and smooth background. This is because system has capture enough signal after Time-lapse choose final images as your best results.
*終成像會有好的信號強(qiáng)度和白色穩(wěn)定的背景。這是因?yàn)?ldquo;Time-lapse”，系統(tǒng)捕捉了足夠多的信號。選擇*后的圖像作為你*好的結(jié)果。
(9) Press reset to next time capture 選擇“Reset”（重設(shè)）開始下次捕捉
De-select Auto-save images when fininsh time-lapse
當(dāng) “time-lapse” 結(jié)束時(shí)，取消“Auto-save images”(自動(dòng)保存圖像)
De-select Auto-stretch 取消“Atuo-stretch”
Set desire 設(shè)定期望值
Black no. 黑度
White no. 白度
Will show image intensity increase gradually. 圖像強(qiáng)度逐漸增加
(10) stop Loop. Start “Time lapse” 停止“Loop”。開始“Time-lapse”
(11) increase exp.start 增加曝光時(shí)間
During Time-lapse capture user can add any no. of imges or expand exp. Time before the last image reach. If you feel sample is too weak.You can stop any time.If you feel sample signal is too strong then just few images is enough.
在“Time-lapse”時(shí)，捕捉*后圖像前使用者都可以增加圖像數(shù)量或者改變曝光時(shí)間。如果你感覺樣品信號太弱，任何時(shí)間都可以停止。如果你感覺太強(qiáng)，少量幾張圖像就夠了。
Under non-auto-stretch control user can see sample signal increase one by one.this will look like X-ray film,but for quantitation user must take care which bands will easy to over expose and saturation.
沒有選擇“auto-stretch”功能的用戶可以觀察到樣品信號一張張?jiān)鰪?qiáng)。這看起來像X光片。但是對于定量用戶必須關(guān)心條帶是否易于曝光和飽和。
User can choose any one image to check results after Time-lapse finished .Just click time-lapse no.
“Time-lapse”后，用戶可以選擇任何一張圖像來核對結(jié)果。只需要點(diǎn)擊對應(yīng)的圖像編碼。
(12) Separate a group of Stack to images into individual image. 從一堆圖像中分出一張獨(dú)立的照片。
(13) Choose “Windows” “Titles” to spread each images on deskTop.
選擇“window”-“Titles”，把每張照片放在桌面上。
(14) Collect all image into a stack file PS:“Stacks”- “imges to stack”-“name”
把所有的圖像放在一個(gè)圖像文件里。
(15) “Make montage”. PS:“Stacks”-“Make montage”
(16) Set scale factor 0.5* or 1* 設(shè)置“scale factor”（比例因子）0.5或0.1
(17) Quantitation: open one images 定量：打開一張圖像
Familiar with the data of chemi-image:Row data should be black background.white bands and intensity will be positive.Most of lab whould like to see the image like X-ray film.so the data will be negative.it need to be inverted when chemi-image what to quantitation.It will not need to inverted.If original image capture with setting at Non-Negative.
熟悉化學(xué)發(fā)光圖像的數(shù)據(jù)：并行數(shù)據(jù)應(yīng)該是黑色背景。白色條帶和亮度較大。大多數(shù)的實(shí)驗(yàn)室想觀察X光片，所以數(shù)據(jù)應(yīng)該是Negative.當(dāng)化學(xué)發(fā)光圖像要定量時(shí)，圖像需要反過來。如果原始圖像設(shè)置為Non-Negative，那么圖像不需要翻轉(zhuǎn)。
The follow image will see data is low when mouse on dark bands and data is high at white background.
當(dāng)鼠標(biāo)移動(dòng)到黑色條帶時(shí)，圖像數(shù)據(jù)較低。白色條帶數(shù)據(jù)較高。
Background correct
Input optimum Rolling ball radius 設(shè)置*佳“Rolling ball radius”值
(18) Choose quantitative tool draw around the target bands. 用定量分析工具（Image J）圈定分析條帶
(19) Press“Analyze”“Measure”repeat for each bands. 選擇“Analyze”-“Measure”對每條條帶重復(fù)操作